王琼

干细胞与再生医学研究组

电话：63846590-776767

邮箱：wangqiong@shsmu.edu.cn  

  干细胞是一类具有自我复制和无限扩增能力的多潜能细胞。根据其产生的部位和分化能力，可分为胚胎干细胞和成体干细胞。胚胎干细胞具有分化成多种类型的细胞和再生各种组织器官的功能。而成体干细胞具有分化成来源器官的各种细胞类型的能力。阐明干细胞多能性和分化的调控信号通路是了解其生理功能和病理机制的前提条件。本课题组主要使用基因组学、表观遗传学和计算生物学等方法，并结合干细胞、类器官和动物等多种模型研究干细胞在生理或病理情况下的分化调控机制，最终促进干细胞的临床应用。主要研究方向：1. 胚胎干细胞向中内胚层分化的调控机制。2. 中内胚层成体干细胞的分化调控和临床应用。

  王琼，yl23411永利研究员，博士生导师。干细胞与再生医学课题组组长，同时是细胞分化与凋亡教育部重点实验室、上海市生殖医学重点实验室、上海市细胞稳态调控与疾病前沿科学研究基地课题组组长。2010年在美国罗切斯特大学Dirk Bohmann教授指导下获得生物医学遗传学博士学位。随后在美国纪念斯隆-凯特琳癌症中心Joan Massague三院院士实验室进行博士后研究。2019年起在yl23411永利组织胚胎学和遗传发育学系担任课题组组长。其实验室主要致力于干细胞的分化调控机制和临床应用的研究，学术论文发表于Cell Stem Cell, Nature  Communications (2), Developmental Cell和Genes & Development等杂志。先后获得纽约干细胞协会（NYSTEM）博后奖，MSKCC Helena Johnson  Hackley学者奖和Geoffrey Beene 癌症研究中心奖，以及上海高校特聘教授（东方学者），全国医药卫生青年科技会议基础医学组一等奖等荣誉，并获得科技部国家重点研发计划，国家自然科学基金和上海市科委等多项基金资助。

1. 细胞周期和DNA甲基化在多能性建立、维持和退出过程中的功能和机制研究，国家重点研发计划，2021-12~2026-06，208万元，课题骨干

2. 胚胎干细胞向中内胚层分化的早期命运决定机制，国家自然科学基金面上项目，2021-01~2024-12，58万元，课题负责人

3. 人胰腺前体细胞的类器官构建与分化发育机制研究，上海市科委面上项目，2020-07~2023-06   20万元 课题负责人

4. TGFβ-SMAD信号通路对干细胞分化的调控机制，国家自然科学基金面上项目，2018-01~2021-12  63万元 课题负责人

5. 上海交大新进青年教师启动计划 2018-07~2020-12  20万元 课题负责人

6. yl23411永利基金引进人才启动基金2018-01~2023-08   200万元 课题负责人

1. Zhao   B., Yu X., Shi J., Ma S., Li S., Shi H., Xia S., Ye Y., Zhang Y., Du Y. &   Wang Q. A stepwise mode of TGFβ-smad signaling and DNA   methylation regulates naive-to-primed pluripotency and differentiation. Nature Communications 2024. 15, 10123. (Last Correspondence, IF=14.7)

2. Huang   H., Jiang Y., Liu J., Luo D., Yuan J., Mu R., Yu X., Sun D., Lin J., Chen Q.,   Li X., Jiang M., Xu J., Chu B., Yin C., Zhang L., Ye Y., Cao B., Wang Q*. & Zhang Y*. Jag1/2 maintain esophageal homeostasis and   suppress foregut tumorigenesis by restricting the basal progenitor cell pool. Nature Communications 2024. 15, 4124. (*Co-correspondence, IF=14.7)

3.  Wang Q., Zou Y., Nowotschin   S., Kim S., Li Q. V., Soh C.-L., Su J., Zhang C., Shu W., Xi Q., Huangfu D.,   Hadjantonakis A.-K., and Massagué, J. The p53 Family Coordinates Wnt and Nodal Inputs in   Mesendodermal Differentiation of Embryonic Stem Cells. Cell  Stem Cell. 2017; 20: 70-86 (First author, with Preview，IF=23.29)

4. Wang Q., Uhlirova M., and  Bohmann D. Spatial restriction of FGF signaling by a matrix   metalloprotease controls branching morphogenesis. Developmental Cell. 2010; 18: 157–164 (First author, Cover article，IF=13.946)

5. Aragón E.#, Wang Q.#, Zou Y.#, Morgani S., Ruiz L., Kaczmarska Z., Su J., Torner C.,   Tian L., Hu J., Shu W., Agrawal S., Gomes T., Márquez J., Hadjantonakis A.,  Macias M., and Massagué J. Structural basis for   distinct roles of SMAD2 and SMAD3 in FOXH1 pioneer-directed TGF-β signaling. Genes & Development. 2019; 33,  1506-1524 (# co-first author，IF=9.527)

6. Wang J., Wang Q..  Establishment of a mouse embryonic stem cell line carrying a reporter of mT-F2A-EGFP based on CRISPR/Cas9n technology. Journal of Shanghai Jiao  Tong University (Medical Science). 2023, 43(4): 417 -427. (Last  Correspondence)

7. Zhao B., Ma S., Xu C., Wang Q.. Different roles of SMAD2 and SMAD3 in human embryonic   stem cell differentiation. Journal of Shanghai Jiao Tong University (Medical Science). 2022, 42(4): 443 -454. (Last  Correspondence)

8. Ma S., Zhao B., Wang Q.. The development and application of pancreatic organoid technology. Chinese Journal of Cell Biology. 2021, 43(6):   1166-1173 (Last Correspondence, Invited Review)

9. Wang X., Wang P., Pan   Y., Wang Q.*, Yin M*. Effect  and mechanism of phenotypic transformation of vascular smooth muscle cells   regulated by TGF-β2 on the venous thrombus wall remodeling. Medical Journal of Chinese People’s Liberation Army. 2020; 45, 791-797. (* Co-correspondence,   IF=0.35)

10. Xu, C., Li, C., Chen, J. Xiong Y., Qiao Z., Fan P., Li C., Ma S., Liu J., Song   A., Tao B., Xu T., Xu W., Chi Y., Xue J., Wang P., Ye D., Gu H., Zhang P., Wang Q., Xiao R., Cheng J., Zheng H., Yu X., Zhang Z., Wu J., Liang K.,   Liu Y., Lu H., Chen F. R-loop-dependent promoter-proximal termination ensures genome stability. Nature. 2023, 621, 610–619.   (IF=64.8)

11. Su J., Morgani S.M., David C.J., Wang Q., Er E.E., Huang Y.H., Basnet H., Zou Y., Shu W., Soni R.K.,   Hendrickson R.C., Hadjantonakis A.K. & Massague J. TGF-beta orchestrates   fibrogenic and developmental EMTs via the ras effector RREB1. Nature. 2020; 577, 566-571. (IF=43.07)

12. Li Q., Dixon G., Verma N., Rosen   B., Gordillo M., Luo R., Xu C., Wang Q., Soh C., Xiang Q.,   Leonardo T., Evan T., Massague J., Garippa R., Huangfu D. Genome-scale screens identify   JNK-JUN signaling as a barrier for pluripotency exit and endoderm   differentiation. Nature Genetics. 2019; 51, 999–1010 (IF=27.959)

13. Martin-Malpartida P.,   Batet M., Kaczmarska Z., Freier R., Gomes T., Aragón Eric, Zou Y., Wang Q., Xi Q., Ruiz L., Vea A., Márquez J., Massagué J. & Macias M. Structural   Basis for Genome Wide Recognition of 5-bp GC Motifs by SMAD Transcription Factors. Nature Communications. 2017; 8(1): 2070-84. (IF=12.124)

14. Gao F.H., Wang Q., Wu Y.L., Li X., Zhao K.W., Chen G.Q. c-Jun N-terminal kinase mediates   AML1-ETO protein-induced connexin-43 expression. Biochemical and Biophysical Research Communications. 2007; 356: 505–511 (IF=2.466)

15. Li X., Xu Y.B., Wang Q., Lu Y., Zheng Y., Wang Y.C., Lubbert M., Zhao K.W., Chen G.Q. Leukemogenic   AML1-ETO fusion protein upregulates expression of connexin 43: The role in   AML1-ETO-induced growth arrest in leukemic cells. Journal of Cellular Physiology. 2006; 208(3): 594 – 01 (IF=4.08)

16. Song M.G., Gao S.M., Du K.M., Xu M., Yu Y., Zhou Y.H., Wang Q., Chen Z., Zhu Y.S. and Chen G.Q. Nanomolar   Level of NSC606985, a camptothecin analogue, induces leukemic cell apoptosis by protein kinase Cd-dependent mechanisms. BLOOD. 2005;  105(9): 3714-21 (IF=13.164)

17. Chen G.Q., Wang Q., Yan H. and Chen Z. Arsenic trioxide and leukemia: from bedside to bench.   (Chapter 11, p251-272) Natural Products: Drug Discovery and Therapeutic Medicine. 2005; Eds Humana Press Inc. 999, Totowa, NJ 0751

 有梦想，有激情，热爱挑战，拒绝躺平，拼搏向上，绝不放弃！